Polyacrylamide gel electrophoresis stained with Coomassie brilliant blue of bacterial cells transformed with pBAD/TOPO^® ThiofusionTM Expression System (ThermoFisher™) which expresses part of the EcCLP1 as a protein fused to thioredoxin (Thio-EcCLP1-his). Lane 1. Non-induced E. coli bacterial cells at 37 °C. Lane 2. Bacterial cells induced with 0.2% of arabinose at 37 °C. Positions of the molecular marker proteins with the corresponding masses in kilodaltons (kDa) are indicated. The arrow shows the recombinant-induced protein.