Open Access
Table 1
Primers used in this study.
| Primer name | Primer sequence 5′ → 3′ | PCR conditions °C/s |
No. of cycles | Length of PCR product [bp] | ||
|---|---|---|---|---|---|---|
| Denaturation | Annealing | Extension | ||||
| EF | GAAACTGCGAATGGCTCATT | 92/45 | 55/45 | 72/90 | 35 | 1500 |
| ER | CTTGCGCCTACTAGGCATTC | |||||
| HepF300 | GTTTCTGACCTATCAGCTTTCGACG | 94/45 | 61/45 | 72/60 | 581 | |
| HepR900 | CAAATCTAAGAATTTCACCTCTGAC | |||||
| Hemo1 | TATTGGTTTTAAGAACTAATTTTATGATTG | 94/45 | 51/45 | 72/90 | 900 | |
| Hemo2 | CTTCTCCTTCCTTTAAGTGATAAGGTTCAC | |||||
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