Open Access

Table 1

Primers used in this study.

Primer name Primer sequence 5′ → 3′ PCR conditions °C/s
No. of cycles Length of PCR product [bp]
Denaturation Annealing Extension
EF GAAACTGCGAATGGCTCATT 92/45 55/45 72/90 35 1500
ER CTTGCGCCTACTAGGCATTC
HepF300 GTTTCTGACCTATCAGCTTTCGACG 94/45 61/45 72/60 581
HepR900 CAAATCTAAGAATTTCACCTCTGAC
Hemo1 TATTGGTTTTAAGAACTAATTTTATGATTG 94/45 51/45 72/90 900
Hemo2 CTTCTCCTTCCTTTAAGTGATAAGGTTCAC

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