Anticoagulant activity of SmANX 18. (a) The formation of fibrin filaments in blood coagulation. (b) Analysis of the anticoagulant activity of SmANX 18 by the coagulation time test (CT). Normal saline and MBP protein were used as controls. Each point is the mean of two replicates ± standard deviation. (c) Changes in the plasma state during blood coagulation. Fibrin was formed after adding calcium ions. (d) The anticoagulant activity assay of SmANX 18 by aPTT. Normal saline and MBP protein were used as controls. The experiments were performed three times. ****p < 0.001, **p < 0.05, ns represents no significant difference.