ANX gene expression of S. mansoni in different stages by qRT-PCR. The expression level was normalized to that of GAPDH and measured with the 2^−ΔΔCt method. The results were averaged from three independent replicates during all stages. Error bars represent SD (n = 3). IMP, immature proglottid; MP, mature proglottid; GP, gravid proglottid. Blue represents plerocercoid; red represents IMP; purple represents MP; green represents GP; orange represents egg.