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Annexin protein family members identified in Spirometra mansoni. (a) Multiple alignment of protein sequences of SmANXs; predicted α-helices are indicated in red. The four-repeat domains of the annexin sequence are indicated with blue dotted boxes. (b) Phylogenetic tree and conserved motifs of SmANXs. (c) Visualization of the SmANX protein model. Each color represents one “annexin repeat”, and each annexin repeat consists of 5 α-helices containing calcium ion binding sites between the fourth and fifth α-helices, called the endonexin fold. (d) Model quality evaluation of the 3D structure of SmANX. Red represents the most favored regions, yellow indicates additional allowed regions, pink indicates the generously allowed regions, and light color indicates disallowed regions.

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