Observation under the light microscope (×400) of different forms of cultured Blastocystis sp. in Jones’ medium. Panel A: Different sizes of the vacuolar form. Panel B: Granular form (blue arrow) and cystic form (black arrow). Panel C: Granular form (blue arrow) and vacuolar form (red arrow). Panel D: Granular form (black arrow) and vacuolar form (red arrow) stained with methylene blue. Panel E: Illustrates the process of transformation of vacuolar cells into multivacuolar forms in culture, showing the division of the central vacuole into smaller vacuoles. Panel D: Different aspects of the amoeboid form with the presence of a single or several pseudopodia (red arrow).