Open Access
Table 1
Primer sequences and reaction conditions used in nested PCR amplifications.
| Target Gene | Primer sequences (5′–3′) | Annealing | Reference |
|---|---|---|---|
| SSU rRNA | Gia2029:AAGTGTGGTGCAGACGGACTC | 55 °C | [4] |
| Gia2150c:CTGCTGCCGTCCTTGGATGT | |||
| RH11:CATCCGGTCGATCCTGCC | 59 °C | ||
| RH4:AGTCGAACCCTGATTCTCCGCCCAGG | |||
| tpi | AL3543:AAATIATGCCTGCTCGTCG | 50 °C | [19] |
| AL3546:CAAACCTTITCCGCAAACC | |||
| AL3544:CCCTTCATCGGIGGTAACTT | 50 °C | ||
| AL3545:GTGGCCACCACICCCGTGCC | |||
| gdh | GDH1:TTCCGTRTYCAGTACAACTC | 50 °C | [6] |
| GDH2:ACCTCGTTCTGRGTGGCGCA | |||
| GDH3:ATGACYGAGCTYCAGAGGCACGT | 50 °C | ||
| GDH4:GTGGCGCARGGCATGATGCA | |||
| bg | G7:AAGCCCGACGACCTCACCCGCAGTGC | 58 °C | [18] |
| G759:GAGGCCGCCCTGGATCTTCGAGACGAC | |||
| 2005F:GAACGAACGAGATCGAGGTCCG | 55 °C | ||
| 2005R:CTCGACGAGCTTCGTGTT |
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