Regulatory effects of egr-miR-71 mimics on immune functions of sheep PBMCs. (A) qPCR analysis of the expression of the core components in the LPS/TLR4 pathway after transfected with egr-miR-71 mimics. (B) ELISA analysis of the expression of TIRAP after transfection with egr-miR-71 mimics. (C) qPCR analysis of the expression of cytokines after transfection with egr-miR-71 mimics. (D) Sandwich ELISA analysis of the level of TNF-α after transfection with egr-miR-71 mimics. (E) The level of NO after transfection with egr-miR-71 mimics determined by Griess reagent. (F) qPCR analysis of the expression of the iNOS after transfection with egr-miR-71 mimics. Data are expressed as mean ± sd; *p < 0.05, **p < 0.01. Data for the final analysis are from three independent experiments. NC, negative control; n.s., not significant.