Identification of egr-miR-71 in the exosomes secreted by E. granulosus protoscoleces. (A) Purified protoscoleces. (B) TEM observation of the enriched exosomes (left), NTA analysis of the size of the exosomes (middle) and Western blotting analysis of the exosomes (right). (C) Detection of the abundance of egr-miR-71 in exosomes by reverse transcription-PCR. (D) Detection of biological activity of egr-miR-71 in exosomes. (E) Internalization of the exosomes by sheep PBMCs observed by confocal microscopy. (F) Flow cytometry analysis of the exosome-internalizing sheep PBMCs. Data are expressed as mean ± SD; ***p < 0.001. Data for the final analysis are from three independent experiments. PSC, protoscoleces; NLK, Nemo-like kinase; Wt, a wild type of the NLK 3’UTR; Mut, a mutant type of the NLK 3’UTR; MFI, mean fluorescence intensity; n.s., not significant; NC, negative control.