Samples showing a banding pattern compatible with the VK210 and VK247 genotypes after digestion with the AluI enzyme of the amplified product. Electrophoresis in 2.5% agarose gel visualized under ultraviolet light. (1) 50 bp molecular weight marker; (2–4) positive controls of the VK210, VK247 and P. vivax-like genotypes, respectively; (5) negative control; (6) single sample positive for the VK210 genotype; (7–12) samples positive for the VK247 genotype, with samples 7, 8 and 9 showing atypical morphology in the microscopic analysis.