Open Access

Table 2

List of character states of reproductive organs used for parsimony analysis. A new character state was used to capture the unique wing-like structure associated with the accessory pieces of Cichlidogyrus ophioglossus n. sp. and C. gnomon n. sp. (in bold: shape of accessory piece – o).

Character Character states
Shape of copulatory tube
  1. Straight: penis more or less straight with no strong arching, twisting, looping, or spiralling but can be slightly sinuous or arched.

  2. Straight, thick-walled: same as before but wall of penis present thickening.

  3. Arched: penis strongly arched in one direction, distal portion often held in position by accessory piece.

  4. Looped: penis draws a loop in the shape of a G.

  5. Large loop: penis draws large circle ending in distal portion of accessory piece.

  6. Spiralled: penis draws spiral in large radius.

  7. Spirally coiled: penis draws spiral in small radius in the shape of a helix.

Diameter of copulatory tube
  1. Tubular: penis in the shape of a simple tube.

  2. Widened: penis widened.

  3. Bulbous: penis presents a bulbous portion (outside the basal bulb).

Shape of accessory piece
  1. Simple: elongated accessory piece without additional structures mentioned in the other character states but species with more unique structures such as connecting stalks and caps are also included here.

  2. Furcated: accessory piece present one or more furcations.

  3. Distal hook: Accessory piece ends in a single distal hook.

  4. Distal flap: Accessory piece ends in a single distal flap.

  5. Gutter-like: Accessory piece in the shape of a gutter guiding the penis.

  6. Ribbon-like: Accessory piece is a flattened structure in the shape of a ribbon or drape.

  7. Spirally coiled: Accessory piece in the shape of simple helix.

  8. Looped: Accessory piece draws a loop in the shape of a G.

  9. Reduced: Accessory piece reduced to a thin, string-like structure or absent.

  10. Complex, S-shaped: massive, roughly S-shaped accessory piece that is frequently connected to the heel. The accessory piece has an extension or thickening at the first turn in the proximal half and frequently displays a folded back, straight and pointy, or hook-like distal end, or sometimes additional terminations resulting in a furcate ending with two or three digitations. However, the first turn is never V-shaped or knee-like such as in (l) and the hook-shaped termination is never sickle-like such as in (c).

  11. Complex, C-shaped: complicated roughly C-shaped accessory piece often with finger or hook-shaped outgrowths and marked heel.

  12. Two portions, V-shaped: accessory piece consists of two distinct portions shaped like a V with an expanded knee-like bend.

  13. Two portions, spiralling: accessory piece consists of two distinct portions, large spiral followed by non-spiralled distal portion.

  14. In two parts: accessory piece consists of two distinct, superimposed parts.

  15. Complex with associated wing-like structure: accessory piece with multiple processes and associated wing-like structure.

Shape of vagina
  1. Non-sclerotised: Vagina not sclerotised.

  2. Tubular: Vagina in the shape of a simple tube.

  3. Bulbous: Vagina widened in at least one portion.

  4. Spiralled: tubular vagina that draws a spiral.

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