Reduction in cell junction protein expression in S-G cells caused by Trichomonas tenax. (A) Western blotting results of cell junctions (ZO-1, JAM-A, E-cadherin, β-catenin, desmoplakin 1 & 2, and connexin) expression levels of S-G cells stimulated by T. tenax at MOI 4 for 24 h. (B) Relative quantification of western blotting results was performed with ImageJ (*p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, MOI: multiplicity of infection).