Open Access
Table 2
Primers and hydrolysis probes designed based on GenBank reference sequences for the targeted parasite detection by qPCR.
| Target species | Target gene | Primer and probe | GenBank reference No (country of origin of the genotyped specimen) | Oligonucleotide sequence (5′–3′) | Probe label | Target size (bp) | References |
|---|---|---|---|---|---|---|---|
| Echinococcus multilocularis | Cytochrome b | Em_cytb_Fwd | AB018440 (Japan) [28] | CGAAAATCCACCAACCACATACT | 84 | This study | |
| Em_cytb_Rev | GCTGCCACTGTCCTTACTTCAA | ||||||
| Em_cytb_Probe | ACCATAGAACCAACCAACGGCAAACTATCA | FAM-TAMRA | |||||
| Echinococcus granulosus s.s. | Cytochrome oxidase subunit III | Eg_cox3_Fwd | AF297617 (for G1) (United Kingdom) [22] | TATCTGTAACACCACAAAACTCAAACC | 149 | This study | |
| Eg_cox3_Rev | CGTTGGAGATTCCGTTTGTTG | ||||||
| Eg_cox3_Probe | AACAAAAGCAAATCACAACAACGTCAACCC | CY5-TAMRA | |||||
| Echinococcus canadensis | NADH dehydrogenase subunit V | Ec_nad5_Fwd | AB208063 (for G6) (Kazakhstan) [26] | ATACGCCATGACTTATCAACTGAAAT | 105 | This study | |
| Ec_nad5_Rev | AB235847 (for G7) (Poland) [26] | AGATTGTGGCTTTGTCAACTTGTAA | |||||
| Ec_nad5_Probe | TCACCAAAAATCAAGTACAAAACGCACCAA | FAM-TAMRA | |||||
| Echinococcus ortleppi | ATP synthase membrane subunit VI | Eo_atp6_Fwd | AB235846 (Argentina) [26] | CTTTTTAGTGTGTATAGCTGAGTCCATTAGT | 145 | This study | |
| Eo_atp6_Rev | CAACGCCCACCAACAACTTA | ||||||
| Eo_atp6_Probe | CCCCATAGTGTTGATTTTGCGTCCTTTCA | CY5-TAMRA | |||||
| Taenia solium | NADH dehydrogenase subunit iV | Tsol_nad4_Fwd | AB086256 (China) [27] | CCCAAAAACGGAACGACAA | 106 | This study | |
| Tsol_nad4_Rev | GCTGTGCATTATCTGTATCTTTTTTAATTG | ||||||
| Tsol_nad4_Probe | ATGCTAATCAACGCTTCCCATCTAACTCGC | HEX-TAMRA | |||||
| Toxocara spp. | Cytochrome oxidase subunit I | Toxo_spp_cox1_Fwd | AM411108 (for T. canis) (China) [23] | AAAATAGCCAARTCCACWCTMCTACCA | 79 | This study | |
| Toxo_spp_cox1_Rev | AM411622 (for T. cati) (China) [23] | GGTGTGGKACTAGTTGAACTGTGTA | |||||
| Toxo_spp_cox1_Probe | CCCCATAGTCCTCAAAG | FAM-MGB | |||||
| Alea – PCR inhibior test | Random sequence | Alea_Fwd | [21] | CCTAAAAATGTCTATGATTGGTCCACTA | 167 | Knapp et al. [21] | |
| Alea_Rev | GGGAGTACCTTGCCATACAAAATT | ||||||
| Alea_probe | TTAAATCAACTCCTAAATCCGCGCGATAGG | VIC-TAMRA |
Current usage metrics show cumulative count of Article Views (full-text article views including HTML views, PDF and ePub downloads, according to the available data) and Abstracts Views on Vision4Press platform.
Data correspond to usage on the plateform after 2015. The current usage metrics is available 48-96 hours after online publication and is updated daily on week days.
Initial download of the metrics may take a while.