Open Access
Research Article

Table 1

Comparison of the sensitivity of conventional PCR (cPCR) and qPCR from the entire dataset of human samples (n = 288). Evaluation of the success of Blastocystis detection by next-generation sequencing (NGS) only in a set of qPCR-positive samples (n = 83).

Sample No. Methods
Sample No. Methods
cPCR qPCR Ct value NGS cPCR qPCR Ct value NGS
B1 + + 15 + B2 + + 19 +
B13 + + 15 + B19 + + 19 +
B24 + + 15 + B115 + + 19 +
B59 + + 15 + B126 + + 19 +
B68 + + 15 + B184 + + 19 +
B195 + + 15 + B220 + + 19 +
B201 + + 15 + B374 + + 19 +
B226 + + 15 + B417 + + 19 +
B235 + + 15 + B86 + + 20 +
B312 + + 15 + B292 + + 20 +
B339 + + 15 + B277 + + 21
B371 + + 15 + B303 + + 21 +
B373 + + 15 + B380 + + 21 +
B9 + + 16 + B300 + + 22 +
B37 + + 16 B375 + + 22 +
B42 + + 16 + B418 + + 22 +
B45 + + 16 + B424 + + 22 +
B49 + + 16 + B431 + + 23 +
B120 + + 16 + B33 + + 24 +
B225 + + 16 + B36 + + 24 +
B327 + + 16 + B313 + + 24 +
B343 + + 16 + B365 + + 24 +
B352 + + 16 + B55 + + 26 +
B364 + + 16 + B144 + 28 +
B412 + + 16 + B345 + + 28 +
B15 + + 17 + B405 + + 29 +
B30 + + 17 + B356 + + 30
B65 + + 17 + B372 + 31 +
B82 + + 17 + B10 + 32
B99 + + 17 + B35 + 32
B113 + + 17 + B38 + 32
B185 + + 17 + B41 + 32 +
B336 + + 17 + B50 + 32
B341 + + 17 + B54 + 32
B353 + + 17 + B62 + 32
B363 + + 17 + B114 + 32 +
B31 + + 18 B189 + 32
B224 + + 18 + B240 + 32 +
B231 + + 18 + B248 + 32
B393 + + 18 B398 + 32
B397 + + 18 + B425 + + 32 +
B413 + + 18 +          

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