Open Access
Table 1
Comparison of the sensitivity of conventional PCR (cPCR) and qPCR from the entire dataset of human samples (n = 288). Evaluation of the success of Blastocystis detection by next-generation sequencing (NGS) only in a set of qPCR-positive samples (n = 83).
| Sample No. | Methods |
Sample No. | Methods |
||||||
|---|---|---|---|---|---|---|---|---|---|
| cPCR | qPCR | Ct value | NGS | cPCR | qPCR | Ct value | NGS | ||
| B1 | + | + | 15 | + | B2 | + | + | 19 | + |
| B13 | + | + | 15 | + | B19 | + | + | 19 | + |
| B24 | + | + | 15 | + | B115 | + | + | 19 | + |
| B59 | + | + | 15 | + | B126 | + | + | 19 | + |
| B68 | + | + | 15 | + | B184 | + | + | 19 | + |
| B195 | + | + | 15 | + | B220 | + | + | 19 | + |
| B201 | + | + | 15 | + | B374 | + | + | 19 | + |
| B226 | + | + | 15 | + | B417 | + | + | 19 | + |
| B235 | + | + | 15 | + | B86 | + | + | 20 | + |
| B312 | + | + | 15 | + | B292 | + | + | 20 | + |
| B339 | + | + | 15 | + | B277 | + | + | 21 | − |
| B371 | + | + | 15 | + | B303 | + | + | 21 | + |
| B373 | + | + | 15 | + | B380 | + | + | 21 | + |
| B9 | + | + | 16 | + | B300 | + | + | 22 | + |
| B37 | + | + | 16 | − | B375 | + | + | 22 | + |
| B42 | + | + | 16 | + | B418 | + | + | 22 | + |
| B45 | + | + | 16 | + | B424 | + | + | 22 | + |
| B49 | + | + | 16 | + | B431 | + | + | 23 | + |
| B120 | + | + | 16 | + | B33 | + | + | 24 | + |
| B225 | + | + | 16 | + | B36 | + | + | 24 | + |
| B327 | + | + | 16 | + | B313 | + | + | 24 | + |
| B343 | + | + | 16 | + | B365 | + | + | 24 | + |
| B352 | + | + | 16 | + | B55 | + | + | 26 | + |
| B364 | + | + | 16 | + | B144 | − | + | 28 | + |
| B412 | + | + | 16 | + | B345 | + | + | 28 | + |
| B15 | + | + | 17 | + | B405 | + | + | 29 | + |
| B30 | + | + | 17 | + | B356 | + | + | 30 | − |
| B65 | + | + | 17 | + | B372 | − | + | 31 | + |
| B82 | + | + | 17 | + | B10 | − | + | 32 | − |
| B99 | + | + | 17 | + | B35 | − | + | 32 | − |
| B113 | + | + | 17 | + | B38 | − | + | 32 | − |
| B185 | + | + | 17 | + | B41 | − | + | 32 | + |
| B336 | + | + | 17 | + | B50 | − | + | 32 | − |
| B341 | + | + | 17 | + | B54 | − | + | 32 | − |
| B353 | + | + | 17 | + | B62 | − | + | 32 | − |
| B363 | + | + | 17 | + | B114 | − | + | 32 | + |
| B31 | + | + | 18 | − | B189 | − | + | 32 | − |
| B224 | + | + | 18 | + | B240 | − | + | 32 | + |
| B231 | + | + | 18 | + | B248 | − | + | 32 | − |
| B393 | + | + | 18 | − | B398 | − | + | 32 | − |
| B397 | + | + | 18 | + | B425 | + | + | 32 | + |
| B413 | + | + | 18 | + | |||||
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