Immunofluorescence localization assay of GSTO2 protein expressed in F. hepatica. (A) Adult sections were incubated with mouse anti-rGSTO2 IgG (1:200) and subsequently incubated with FITC-conjugated goat anti-mouse IgG (1:1500 dilutions). (B) The same sections as column (A) were incubated with naïve mouse serum (1:200, negative control) and then incubated with FITC-conjugated goat anti-mouse IgG (1:1500 dilutions). (C) HE staining of sections of adult F. hepatica, scale-bars: 50 μm.