Open Access
Table 1
Primer sequences used for the gene amplification of different pathogens.
| Pathogen gene | Primer name | Sequence (5′–3′) | Annealing temperature (°C) | Fragment size (bp) | Reference |
|---|---|---|---|---|---|
| CRT ompA | Rr190.70p | ATGGCGAATATTTCTCCAAAA | 60 | 346 | Jia et al. [4] |
| Rr190.602n | AGTGCAGCATTCGCTCCCCCT | ||||
| 190.70-38s1 | AAAACCG CTTTATTCACC | 58 | |||
| 190.602-384r1 | GGCAAC AAGTTACCTCCT | ||||
| CRT 17kDa | 17K3 | GCTTTACAAAATTCTAAAAACCATATA | 50 | 395 | Jia et al. [4] |
| 17K5 | TGTCTATCAATTCACAACTTGCC | ||||
| 17KD113s1 | ATTGTCCGTCAGGTTGGC | 52 | |||
| 17KD408r1 | CGGGCGGTATGAATAAGC | ||||
| SFGR Candidatus Rickettsia longicornii ompA | H-LompA-F | TTTAATTGATTTAATTTTTATTAAGGTTTACATATGGCG | 60 | 647 | Jiang et al. [3] |
| H-LompA-R | GTCTTGACAGTTATTATACCTCCTCCAT | ||||
| SFGR Candidatus Rickettsia longicornii ompB | H-LompB-F1 | GTTCAGCTATGGGTGCTGCTATACAG | 63 | 1203 | Jiang et al. [3] |
| H-LompB-R1 | GCACTAGCACTTGCTAAAGTACCGT | ||||
| SFGR Candidatus Rickettsia longicornii sca4 | H-Lsca4-F2 | AGTTCTCAGTCCAGCACAACAAC | 63 | 885 | Jiang et al. [3] |
| H-Lsca4-R2 | GCCTTTACCAGCTCATCTACTTT | ||||
| SFGR Candidatus Rickettsia longicornii rrs | H-L16S-F | TGCAAGTCGAACGGACTAATTGG | 65 | 976 | Jiang et al. [3] |
| H-L16S-R | AATGAGGGTTGCGCTCGTTG | ||||
| SFTSV Small | S-F1 | ACACAAAGACCCCCTTCATTTGGA | 58 | 588 | Liu et al. [10] |
| S-R1 | TGGAGGAGGGCCACATCCAG | ||||
| SFTSV medium | M-F1 | GATGAGATGGTCCATGCTGATTCT | 58 | 560 | Liu et al. [10] |
| M-R1 | CTCATGGGGTGGAATGTCCTCAC | ||||
| SFTSV large | L-F1 | ACACAGAGACGCCCAGATGAAC | 60 | 684 | Liu et al. [10] |
| L-R1 | GCCTCAAGCTCTTCCTCACTCTTCTG | ||||
| T. orientalis MPSP | P1 | CACGCTATGTTGTCCAAGAG | 53 | 875 | Ota et al. [15] |
| P2 | TGTGAGACTCAATGCGCCTA | ||||
| T. sinensis MPSP | P3 | CACTGCTATGTTGTCCAAGAGATATT | 56 | 887 | Liu et al. [8] |
| P4 | AATGCGCCTAAAGATAGTAGAAAAC |
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