Open Access
Table 4
Review of previous comparative studies of commercial multiplex PCR panels targeting gastrointestinal protists.
Reference | PCR method | Study design | Samples (n) | DNA extraction | Amplification technologies | Gold standard | Protozoa Panel | Se (%) | Sp (%) |
---|---|---|---|---|---|---|---|---|---|
[23] | Easyscreen | Prospective | 358 | EZ1® (Tissue kit and card) | Taqman on Cepheid® smart cycler | Microscopy + PCR | Blastocystis sp. | 96 | 100 |
Cryptosporidium spp. | 100 | 100 | |||||||
D. fragilis | 95 | 100 | |||||||
Entamoeba spp. | 92 | 100 | |||||||
G. intestinalis | 92 | 100 | |||||||
[12] | G DiaParaTrio | Retrospective | 185 | QIAsymphony® | Taqman on RotorGene | Microscopy + PCR | C. hominis/C. parvum | 96 | 100 |
E. histolytica | 100 | 100 | |||||||
G. intestinalis | 92 | 100 | |||||||
[1] | G DiaParaTrio | Prospective | 90 | MagNA Pure 96 | Taqman and melt curve on Light Cycler 480 | Microscopy | Cryptosporidium spp. | 55–100 | 100 |
Ridagene | G. intestinalis | 41–89 | 95–98 | ||||||
BD max EPP | BD max | BD max | D. fragilis | 71 | 97 | ||||
[18] | G DiaParaTrio | Retrospective | 126 | QIAamp DNA stool mini kit | Taqman on RotorGene | PCR + Sanger sequencing | Cryptosporidium spp. | 53.1–87.5 | NA |
Ridagene | Speedtools DNA extraction | Taqman and melt curve on Mx3005P | E. histolytica | 0–100 | NA | ||||
DNA Extract-VK | |||||||||
Allplex GI | Powerfecal DNA isolation | MuDT on CFX-96 | G. intestinalis | 68–100 | NA | ||||
FTD stool parasites | Wizard magnetic DNA purification | ||||||||
[2] | Allplex GI | Retrospective | 103 | StarLab carry blair medium | MuDT on CFX-96 | Microscopy | Blastocystis sp. | 98.2–100 | NA |
Cryptosporidium spp. | 100 | NA | |||||||
C. cayetanensis | 100 | NA | |||||||
Prospective | 588 | D. fragilis | 80–81 | NA | |||||
E. histolytica | NA | NA | |||||||
G. intestinalis | 81–100 | NA | |||||||
[14] | BD max EPP | Retrospective | 391 | BD max | BD max | Microscopy + PCR | C. hominis/C. parvum | 95.5 | 99.6 |
Prospective | 2104 | E. histolytica | 100 | 100 | |||||
G. intestinalis | 98.2 | 99.5 |
Abbreviations: FTD: fast track diagnosis, GI: gastrointestinal, EPP: enteric parasite pathogen, Se: sensitivity, Sp: specificity.
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