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Table 4

Review of previous comparative studies of commercial multiplex PCR panels targeting gastrointestinal protists.

Reference PCR method Study design Samples (n) DNA extraction Amplification technologies Gold standard Protozoa Panel Se (%) Sp (%)
[23] Easyscreen Prospective 358 EZ1® (Tissue kit and card) Taqman on Cepheid® smart cycler Microscopy + PCR Blastocystis sp. 96 100
Cryptosporidium spp. 100 100
D. fragilis 95 100
Entamoeba spp. 92 100
G. intestinalis 92 100
[12] G DiaParaTrio Retrospective 185 QIAsymphony® Taqman on RotorGene Microscopy + PCR C. hominis/C. parvum 96 100
E. histolytica 100 100
G. intestinalis 92 100
[1] G DiaParaTrio Prospective 90 MagNA Pure 96 Taqman and melt curve on Light Cycler 480 Microscopy Cryptosporidium spp. 55–100 100
Ridagene G. intestinalis 41–89 95–98
BD max EPP BD max BD max D. fragilis 71 97
[18] G DiaParaTrio Retrospective 126 QIAamp DNA stool mini kit Taqman on RotorGene PCR + Sanger sequencing Cryptosporidium spp. 53.1–87.5 NA
Ridagene Speedtools DNA extraction Taqman and melt curve on Mx3005P E. histolytica 0–100 NA
DNA Extract-VK
Allplex GI Powerfecal DNA isolation MuDT on CFX-96 G. intestinalis 68–100 NA
FTD stool parasites Wizard magnetic DNA purification
[2] Allplex GI Retrospective 103 StarLab carry blair medium MuDT on CFX-96 Microscopy Blastocystis sp. 98.2–100 NA
Cryptosporidium spp. 100 NA
C. cayetanensis 100 NA
Prospective 588 D. fragilis 80–81 NA
E. histolytica NA NA
G. intestinalis 81–100 NA
[14] BD max EPP Retrospective 391 BD max BD max Microscopy + PCR C. hominis/C. parvum 95.5 99.6
Prospective 2104 E. histolytica 100 100
G. intestinalis 98.2 99.5

Abbreviations: FTD: fast track diagnosis, GI: gastrointestinal, EPP: enteric parasite pathogen, Se: sensitivity, Sp: specificity.

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