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Table 1

Pathogens included to evaluate the analytical performances of multiplex PCRs, identified by microscopy (iodine-stained wet mount, Bailanger’s method and modified Ziehl–Neelsen staining) completed by E. histolytica-specific adhesion for amoeba species only (composite reference method) [12].

Pathogen species identified by the reference method Number of stool samples (n = 134)
Blastocystis sp. 17
Chilomastix mesnili 2
Cryptosporidium hominis 6
Cryptosporidium parvum 20
Cryptosporidium felis 1
Cryptosporidium meleagridis 1
Cystoisospora belli 2
Dicrocoelium dendriticum 1
Dientamoeba fragilis 1
Endolimax nana 21
Entamoeba coli 35
Entamoeba dispar/moshkovskii 12
Entamoeba hartmanni 4
Entamoeba histolytica 4
Enterocytozoon bieneusi 1
Giardia intestinalis 37
Hookworms 2
Hymenolepis nana 2
Iodamoeba butschlii 3
Pentatrichomonas intestinalis 1
Sarcocystis spp. 1
Schistosoma mansoni 4
Taenia spp. 1
Trichuris trichiura 1
Negative 50

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