Open Access
Table 4
PCR results according to Echinococcus species and sample type. N: number of samples tested, n: number of positive samples, *53 AE FFPE samples tested instead of 54, Se: sensitivity, and Sp: specificity, Em: E. multilocularis, Eg: E. granulosus, E spp.: Echinococcus spp.
| Target gene | 16S-rrnL-rRNA | 12S-rrnS rRNA | nad1 | 12S-rrnS rRNA | nad1 | 12S-rrnS rRNA | 12S-rrnS rRNA | nad1 | cox1 | nad1 | |
|---|---|---|---|---|---|---|---|---|---|---|---|
| PCR name | A | B1 | C | D | E | F | G | B2 | H | I | |
| 16S | Trachsel EG | JB11.5 | EMH15 | EM29 | EG1 | 12S | Trachsel EM | COX1 | JB11 | ||
| Target species | Em | Eg | E spp. | Em | Em | Eg | E spp. | Em | E spp. | E spp. | |
| PCR product size (bp) | 84 | 117 | 183 | 200 | 252 | 255 | 268 | 395 | 446 | 529 | |
| AE frozen (N = 20) | Se (n) | 100% (20) | / | 95.0% (19) | 100% (20) | 100% (20) | 0% (0) | 100% (20) | 100% (20) | 60.0% (12) | 100% (20) |
| CE frozen (N = 5) | Se (n) | 0% (0) | 100% (5) | 100% (5) | 0% (0) | 0% (0) | 100% (5) | 100% (5) | 0% (0) | 80% (4) | 0% (0) |
| Sp for AE diagnosis | 100% | / | – | 100% | 100% | 100% | – | 100% | – | – | |
| AE FFPE (N = 54) | Se (n) | 69.8% (37*) | / | 1.9% (1) | 33.3% (18) | 33.3% (18) | 0% (0) | 31.5% (17) | 18.5% (10) | 3.7% (2) | 13.0% (7) |
| CE FFPE (N = 10) | Se (n) | 0% (0) | 20% (2) | 0% (0) | 0% (0) | 0% (0) | 0% (0) | 0% (0) | 0% (0) | 0% (0) | 0% (0) |
| Sp for AE diagnosis | 100% | / | – | 100% | 100% | / | – | 100% | – | – | |
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