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Phylogenetic tree based on CSP sequences of Plasmodium spp. All P. knowlesi CSP sequences obtained in this study are annotated as M257, M208 (P-M208-9-M13F, P-M208-3-M13F), M42 (P-M42-21-M13F, P-M42-22-M13F), M145 (P-145-7-M13F, P-145-4-M13F, P-145-3-M13F), M258, M206 (P-206-14-M13F, P-206-10-M13F), M65 (P-65-39-M13F), M260, M243, M222, M248, M264, and M234. The phylogenetic tree was constructed using the NJ method in MEGA7 (A), Bayesian (B) method using MrBayes software, and Maximum likelihood method (C) in MEGA-X. The percentages of replicate trees in which the associated isolates cluster together in the bootstrap test (1000 replicates) are shown next to the branches. The tree was drawn to scale, with branch length in the same unit as those of the evolutionary distances used to infer the phylogenetic tree. The evolutionary distances were computed using the Kimura 2-parameter and are in the units of the number of base substitutions per site.

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