Open Access
Research Article
Table 1
Primers used in this study.
| Gene/region | Primers and sequences 5′–3′ | Cycling conditions | References |
|---|---|---|---|
| 28S | LSU | Initial denaturation of 7 min at 95 °C; 40 cycles of 40 s at 95 °C, 45 s at 57 °C and 1 min and 30 s at 72 °C; 10 min at 72 °C for final extension | [34, 43] |
| TAGGTCGACCCGCTGAAYTTAAGCA | |||
| 1500R | |||
| GCTATCCTGAGGGAAACTTCG | |||
| 1200R | |||
| GCATAGTTCACCATCTTTCGG | |||
| ITS | GLYP1 | Initial denaturation of 7 min at 95 °C; 40 cycles of 40 s at 95 °C, 45 s at 57 °C and 1 min 30 s at 72 °C; 10 min at 72 °C for final extension | [36, 56] |
| GCTGAGAAGACGACCAAACTTGAT | |||
| BD2 | |||
| TATGCTTAAATTCAGCGGGT | |||
| COI | JB3 | Initial denaturation of 5 min at 94 °C; 40 cycles of 30 s at 92 °C, 45 s at 47 °C and 1 min 30 s at 72 °C; 10 min at 72 °C for final extension | [42, 55] |
| TTTTTTGGGCATCCTGAGGTTTAT | |||
| JB4.5 | |||
| TAAAGAAAGAACATAATG AAAATG |
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