Three new species of Creptotrema (Trematoda, Allocreadiidae) with an amended diagnosis of the genus and reassignment of Auriculostoma (Allocreadiidae), based on morphological and molecular evidence

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Table 1

Primers used in this study.

Gene/region	Primers and sequences 5′–3′	Cycling conditions	References
28S	LSU	Initial denaturation of 7 min at 95 °C; 40 cycles of 40 s at 95 °C, 45 s at 57 °C and 1 min and 30 s at 72 °C; 10 min at 72 °C for final extension	[34, 43]
	TAGGTCGACCCGCTGAAYTTAAGCA
	1500R
	GCTATCCTGAGGGAAACTTCG
	1200R
	GCATAGTTCACCATCTTTCGG
ITS	GLYP1	Initial denaturation of 7 min at 95 °C; 40 cycles of 40 s at 95 °C, 45 s at 57 °C and 1 min 30 s at 72 °C; 10 min at 72 °C for final extension	[36, 56]
	GCTGAGAAGACGACCAAACTTGAT
	BD2
	TATGCTTAAATTCAGCGGGT
COI	JB3	Initial denaturation of 5 min at 94 °C; 40 cycles of 30 s at 92 °C, 45 s at 47 °C and 1 min 30 s at 72 °C; 10 min at 72 °C for final extension	[42, 55]
	TTTTTTGGGCATCCTGAGGTTTAT
	JB4.5
	TAAAGAAAGAACATAATG AAAATG

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