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Table 3
Characteristics of serologic methods used for anti-Toxoplasma IgG detection.
| Characteristics | Microplate ELISA | Automated Immuno-assay | HAI | AGGLUTINATION | ICT | WB |
|---|---|---|---|---|---|---|
| Multiparametric | No | Yes | No | No | No | No |
| Turnaround time | 1–3 series per week, depending on the lab activity | Series or on demand, depending on the lab activity | Most suited to series | On demand | Unitary test | Unitary test |
| Suitability for emergency | No | Yes | Possible | Yes | Yes | No |
| Duration of analysis | 3–4 h | 0.5–2.5 h | 2.5 h | 10 min | 15 min | 3 h |
| Storage of reagents | 4–8 °C | 4–8 °C | 4–8 °C | 4–8 °C | RT | 4–8 °C |
| Device | Spectrophotometer* | Automate | None | None | None | Oscillating agitator |
| Maintenance | Yearly | Daily, weekly, monthly, yearly | None | None | None | None |
| Handiness | Skills required++ | Skills required++ | Easy | Easy | Easy | Skills required+ |
| Reading/interpretation | Standardized | Standardized | Touchy | Easy | Easy | Easy |
| Cost | Intermediate | High | Low | Low | Low-Intermediate | High |
| Destination | Reference or district lab | Reference lab | District lab | Local lab | Local lab | Reference or district lab |
RT: room temperature.
*These assays can be automated using a microplate setup device.
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