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Review Article

Table 3

Characteristics of serologic methods used for anti-Toxoplasma IgG detection.

Characteristics Microplate ELISA Automated Immuno-assay HAI AGGLUTINATION ICT WB
Multiparametric No Yes No No No No
Turnaround time 1–3 series per week, depending on the lab activity Series or on demand, depending on the lab activity Most suited to series On demand Unitary test Unitary test
Suitability for emergency No Yes Possible Yes Yes No
Duration of analysis 3–4 h 0.5–2.5 h 2.5 h 10 min 15 min 3 h
Storage of reagents 4–8 °C 4–8 °C 4–8 °C 4–8 °C RT 4–8 °C
Device Spectrophotometer* Automate None None None Oscillating agitator
Maintenance Yearly Daily, weekly, monthly, yearly None None None None
Handiness Skills required++ Skills required++ Easy Easy Easy Skills required+
Reading/interpretation Standardized Standardized Touchy Easy Easy Easy
Cost Intermediate High Low Low Low-Intermediate High
Destination Reference or district lab Reference lab District lab Local lab Local lab Reference or district lab

RT: room temperature.

*These assays can be automated using a microplate setup device.

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