Nucleotide sequence of 529 showing the set of primers and the probe. The sequences marked with red, purple, brown, blue, green, and orange represent primers F3, F2, F1c, B1c, B2, and B3, respectively. The forward inner primer (FIP=F1c−F2) was labeled with biotin at the 5′ end, with amplification in the 5′–3′ direction. The yellow module denotes the sequence of FITC-probe between primer B1c and B2.