Open Access
Table 1
Primer sequences for qRT-PCR analysis of gene transcripts.
| Gene | Primer sequences (5′–3′) | Expected size (bp) | Annealing temperature (°C) |
|---|---|---|---|
| NTNG1 | F: CAAAGACAGGTTCGCATT | 214 | 59.5 |
| R: CCTTCCGTGCACTTTTAT | |||
| EphA3 | F: CCAGCGATGTATGGAGTTA | 180 | 58.5 |
| R: CTTTCTGCCAGCAGTCTAG | |||
| NCOA1 | F: GATCTAACCTCCCTTCTGCTTTT | 228 | 61 |
| R: GGAACAGAGAGGACAACGCA | |||
| SCD | F: CGCTGGCACATCAACTTTACC | 124 | 56.6 |
| R: TTTCCTCTCCAGTTCTTTTCATCC | |||
| GHR | F: AGGTTGCTCAGCCACAAA | 281 | 58.9 |
| R: TGGGGAAAGGACCACATT | |||
| SMAD3 | F: CGACTACAGCCATTCCATCC | 120 | 60.5 |
| R: TGGTCACTGGTCTCTCCATCT | |||
| TGFBR1 | F: ATTCTGTGGCTTTGCCTGAA | 175 | 60 |
| R: GCAGTTTCCTGGGTCTGAAG | |||
| IGF1 | F: GACAGGAATCGTGGATGAGTG | 270 | 57 |
| R: AACAGGTAACTCGTGCAGAGC | |||
| LPL | F: GTCATCGTGGTGGACTGG | 398 | 59.3 |
| R: TGGAAAGTGCCTCCGTTA | |||
| GAPDH | F: CTGACCTGCCGCCTGGAGAAA | 149 | 59.0 |
| R: GTAGAAGAGTGAGTGTCGCTGTT |
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