Open Access
Research Article

Figure 2

image

Download original image

Assessment of the acaricidal potential of the plasma from chickens immunised with the recombinant cysteine protease protein from PRMs (Deg-CPR-1) against poultry red mites (PRMs, Dermanyssus gallinae). (A) The production of antibody in the plasma from chickens immunised with Deg-CPR-1. Four chickens were immunised with Deg-CPR-1 and three chickens were unimmunised. The plasma was isolated from immunised and unimmunised chickens, and the antibody specific to Deg-CPR-1 in the plasma was detected by western blotting. The arrow head indicates the predicted molecular weight of Deg-CPR-1 (63 kDa). (B and C) The mortality rate of PRMs that were fed blood containing the plasma from immunised chickens was assessed every day for 1 week. Four and three plasma samples isolated from the immunised or unimmunised chickens were used in the in vitro feeding assays, and the total number of blood-feeding PRMs monitored was as follows: immunised: n = 155; unimmunised: n = 89 (B). The total number of PRMs fed blood containing the plasma with high antibody titre or low antibody titre was as follows: high antibody titre: n = 74; low antibody titre: n = 81 (C). The number of dead PRMs was recorded and plotted on the graph to generate Kaplan–Meier curves. Statistical analysis was performed using a log-rank test. Results with p < 0.01 were considered statistically significant.

Current usage metrics show cumulative count of Article Views (full-text article views including HTML views, PDF and ePub downloads, according to the available data) and Abstracts Views on Vision4Press platform.

Data correspond to usage on the plateform after 2015. The current usage metrics is available 48-96 hours after online publication and is updated daily on week days.

Initial download of the metrics may take a while.