NO-induced egress of sporozoites is dependent on parasitic mobility. (A) Sporozoite-infected PCKs were pre-treated with 10 μM Cyto-D and then incubated with 40 mM SNP for 30 min. Free sporozoites were analyzed by flow cytometry. Data are means ± SEM of four replicates, representing three independent experiments. **p < 0.01. (B) and (C) Images captured after SNP treatment and pre-incubation with 10 μM Cyto-D. Bar: 20 μm.