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NO-induced egress is required for intra-parasitic Ca 2+ . Sporozoite-infected PCKs were pre-treated with BAPTA-AM (A) or U-73122 (B) for 30 min and then incubated with 40 mM SNP for 30 min. Free parasites were analyzed by flow cytometry. Data are means ± SEM of six replicates, representing three independent experiments. **p < 0.01.

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