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Parasite development is essential for NO-induced egress. PCKs were infected with sporozoites for 12, 24, or 36 h and then incubated with 40 mM SNP for 30 min. The number of free sporozoites was analyzed by flow cytometry. Data are means ± SEM of six replicates, representing three independent experiments. *p < 0.05; **p < 0.01.

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