Open Access
Table 1
Primer sequences and reaction conditions used in nested PCR amplifications.
| Locus | Primer sequences (5′ – 3′) | Nucleotide fragment (bp) | Annealing temperature (°C) | Reference |
|---|---|---|---|---|
| Cryptosporidium spp. SSU rRNA | SSU-F2: TTCTAGAGCTAATACATGCG | ~1325 | 55 | Xiao et al. [37] |
| SSU-R2: CCCATTTCCTTCGAAACAGGA | ||||
| SSU-F3: GGAAGGGTTGTATTTATTAGATAAAG | 826–864 | 55 | ||
| SSU-R4: CTCATAAGGTGCTGAAGGAGTA | ||||
| C. parvum gp60 | AL3531: ATAGTCTCCGCTGTATTC | 1280 | 52 | Alves et al. [1] |
| AL3535: GGAAGGAACGATGTATCT | ||||
| AL3532: TCCGCTGTATTCTCAGCC | 800–850 | 50 | ||
| AL3534: GCAGAGGAACCAGCATC | ||||
| G. duodenalis SSU rRNA | Gia2029: AAGTGTGGTGCAGACGGACTC | 497 | 55 | Appelbee et al. [2] |
| Gia2150c: CTGCTGCCGTCCTTGGATGT | ||||
| RH11: CATCCGGTCGATCCTGCC | 292 | 59 | ||
| RH4: AGTCGAACCCTGATTCTCCGCCCAGG | ||||
| G. duodenalis tpi | AL3543: AAATIATGCCTGCTCGTCG | 605 | 50 | Cacciò and Ryan [4] |
| AL3546: CAAACCTTITCCGCAAACC | ||||
| AL3544: CCCTTCATCGGIGGTAACTT | 530 | 50 | ||
| AL3545: GTGGCCACCACICCCGTGCC |
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