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Figure 3

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Agarose gel electrophoresis of UBE2F ORF and identification of recombinant plasmid pET-32a-UBE2F digested by EcoR I and Xho I. (A) (Lane M) DNA molecular weight marker DL 2000 (ordinate values in bp); (Lane 1) the ORF of UBE2F. (B) (Lane M) DNA molecular weight marker DL 5000 (ordinate values in bp); (Lane 1) the recombinant plasmid pET-32a-UBE2F digested by EcoR I and Xho I; (Lane 2) the recombinant plasmid pET-32a-UBE2F; (Lane 3) the plasmid of pET-32a (+) vector digested by EcoR I and Xho I.

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