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Table 3

Allele frequencies observed at seven microsatellite loci tested in A berlandi, in comparison with those we previously calculated in A. pegreffii and A. simplex (s. s) at the same SSR loci [9]. With regard to the polymorphic sex-linked locus Anisl 7, the most reliable estimate of allele frequencies was calculated according to the sex-linked genetic model estimate, assuming: (i) hemizygosity of males at this locus; (ii) adult female counterparts as biallelic at the sex-linked loci. The frequencies of Anisl 00185 and Anisl 00314 are not shown in A. simplex (s. s.), because these loci were affected by null alleles, as we have previously found [9].

Locus Allele Anisakis berlandi Anisakis pegreffii Anisakis simplex (s. s.)
Anisl 00185 182 0.01
185 0.01 0.01
188 0.01 0.04
191 0.11 0.07
194 0.15 0.25
197 0.18 0.24
200 0.28 0.15
203 0.09 0.18
206 0.07 0.04
209 0.07 0.01
212 0.02 0.01
Anisl 00314 96 0.05
100 1.00 0.32
104 0.22
108 0.25
112 0.13
116 0.01
120 0.01
124 0.01
Anisl 10535 125 0.01 0.01
128 1.00 0.01 0.01
131 0.02 0.02
134 0.10 0.91
137 0.14 0.04
140 0.51 0.01
143 0.18
146 0.02
149 0.01
Anisl 05784 57 0.01
60 0.01
63 0.01 0.21
66 0.01 0.04
69 0.98 0.01 0.03
72 0.02 0.01 0.01
75 0.01 0.02
78 0.02 0.46
81 0.05 0.13
84 0.07 0.05
87 0.22 0.01
90 0.31 0.01
93 0.18
96 0.05 0.01
99 0.03
102 0.01
105 0.01
Anisl 08059 78 0.01
82 0.01 0.03
86 0.80 0.24 0.85
90 0.19 0.03 0.05
94 0.01 0.12 0.01
98 0.13 0.02
102 0.19 0.01
106 0.12 0.01
110 0.06 0.01
114 0.04
118 0.02
122 0.01
126 0.01
130 0.01
134 0.01
Anisl 00875 142 0.01
145 0.01 0.01
148 0.95 0.01 0.01
151 0.04 0.05 0.01
154 0.01 0.03 0.01
157 0.67 0.20
160 0.16 0.53
163 0.04 0.16
166 0.01 0.04
169 0.01
172 0.01 0.01
175 0.01
Anisl 7 216 0.01
219 0.22
222 0.75
225 0.02 0.02
228 0.02
243 0.02
252 0.02 0.13
255 0.48
258 0.02 0.18
261 0.07 0.11
264 0.02 0.04
267 0.20 0.03
270 0.17 0.02
273 0.11
276 0.07
279 0.09
282 0.11
285 0.02 0.01
288 0.02
294 0.02

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