(A) F_IS calculated at the six microsatellite loci among the eight studied in A. berlandi. Two loci (i.e. Anisl 00314 and Anisl 10535) were not included because they were found to be monomorphic. Negative values indicate heterozygous excess, while positive values indicate homozygous excess from that expected under Hardy–Weinberg Equilibrium (HWE); (B) F_IS in male and female specimens of A. berlandi at the sex-linked locus Anisl 7.