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(A) Measurement of cholesterol content in nematode eggs before and after MβCD (2.25 mM) treatment. The cholesterol content of the two susceptible isolates was higher than that of the two resistant isolates (p < 0.05). Treatment of eggs significantly decreased the cholesterol concentration in eggs, except for the HcR-G isolate (p < 0.05). (B) Measurement of phospholipid content in nematode eggs before and after MβCD (2.25 mM) treatment. The phospholipid content was not modified by the MβCD treatment. (C) Measurement of anisotropy after alterations in eggshells. MβCD treatment decreased the anisotropy significantly in HcS-WB, HcS-Ca, and HcR-WR isolates (p < 0.05). Means of lipid concentrations (M ± SD for three measurements). *Significant effect (p < 0.05). Symbols: (grey rectangles) control eggs and (black rectangles) MβCD treatment. HcS-WB: Haemonchus contortus susceptible Weybridge, HcS-Ca: Haemonchus contortus susceptible Canada, HcR-WR: Haemonchus contortus resistant White River, and HcR-G: Haemonchus contortus resistant Guadeloupe.
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