A cardiac and subcutaneous canine dirofilariosis outbreak in a kennel in central France

Open Access

Table 1

Primers and probes used in this study.

System name	Primer & probe name	Sequences 5′–3′	Specificity	References
Pan-fil 28S qPCR-based system	qFil-28S-F	TTGTTTGAGATTGCAGCCCA	Filariae	[22]
	qFil-28S-P	6FAM-5′-CAAGTACCGTGAGGGAAAGT-3′-TAMRA
	qFil-28S-R	GTTTCCATCTCAGCGGTTTC
All-Wol 16S qPCR-based system	all.Wol.16S.301-F	TGGAACTGAGATACGGTCCAG	Wolbachieae
	all.Wol.16S.347-P	6FAM-5′-AATATTGGACAATGGGCGAA-3′-TAMRA
	all.Wol.16S.478-R	GCACGGAGTTAGCCAGGACT
Triplex TaqMan COI qPCR-based system	Fil.COI.749-F	CATCCTGAGGTTTATGTTATTATTTT
	D.imm.COI.777-P	6FAM-CGGTGTTTGGGATTGTTAGTG-TAMRA	Dirofilaria immitis
	D.rep.COI.871-P	6VIC-TGCTGTTTTAGGTACTTCTGTTTGAG-TAMRA	Dirofilaria repens
	A.rec.COI.866-P	Cy5-TGAATTGCTGTACTGGGAACT-BHQ-3	Acanthocheilonema reconditum
	Fil.COI.914-R	CWGTATACATATGATGRCCYCA
Duplex Wol-Diro ftsZ qPCR-based system	WDiro.ftsZ.490-F	AAGCCATTTRGCTTYGAAGGTG	Wolbachia endosymbiont of D. immitis and D. repens
	WDimm.ftsZ.523-P	6FAM-CGTATTGCAGAGCTCGGATTA-TAMRA
	WDrep.ftsZ.525-P	6VIC-CATTGCAGAACTGGGACTGG-TAMRA
	WDiro.ftsZ.600-R	AAACAAGTTTTGRTTTGGAATAACAAT
Duplex HWs COI qPCR-based system	Hw.COI.723-F	TCAGCATTTGTTTTGGTTTTT
	D.imm.COI.777-P	6FAM-CGGTGTTTGGGATTGTTAGTG-TAMRA	D. immitis
	A.vas.COI.813-P	6VIC-TGACTGGGAAGAAGGAGGTG-TAMRA	Angiostrongylus vasorum
	Hw.COI.950-R	GCASTAAAATAAGYACGAGWATC
Pan-Nematoda primers 18S PCR-based system	Fwd.18S.631	TCGTCATTGCTGCGGTTAAA	Nematoda	This study
Pan-Nematoda primers 18S PCR-based system	Rwd.18S.1825r	GGTTCAAGCCACTGCGATTAA	Nematoda	This study

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