Alignment of the amino-acid sequences of the MCA orthologs in different Leishmania species. The N-terminal domain, which contains a mitochondrial localization signal, from amino-acids 1 to 62, is indicated in blue; the catalytic domain, containing the catalytic dyad histidine 147/cysteine 202 (boxed in red), is indicated by a dashed red line; the proline-rich C-terminal domain is indicated by a green line. Demonstrated cleavage sites are boxed in blue: R63, R136, R218 and R298.