Open Access
Table 1
List of Primer used in this experiment.
| Primer name | Sequence (5′–3′) | Product size | Use |
|---|---|---|---|
| DG-F1 | GTIGCIATHAARAARGTIYTICARGAY | 600 bp | For Preliminary identification of GSK-3β |
| DG-R1 | YTTRWRYTCIRTRTARTTIGGRTTCAT | ||
| Orf-F1 | ATGAGTGGACGGCCGAGGACG | 1248 bp | Primer extension |
| Orf-R1 | AGTGGCCCCGTGTAAATAAAG | ||
| GSK-F1 | CGCCTGGACCACTGTAACAT | 351 bp | For transcriptional analysis |
| GSK-R1 | CGAGCAGATGTACGACACGT | ||
| Actin-F1 | AGCGTGGCTACTCTTTCACC | 229 bp | |
| Actin-R1 | GATTCCATACCCAGGAACGA | ||
| T7-GSK-F1 | TAATACGACTCACTATAGGGTACTGCGCTTCAAGAACCGCGAA | 318 bp | For ds RNA synthesis |
| T7-GSK-R1 | TAATACGACTCACTATAGGGTACTTTTAGTACGCCCGTCTCTGG |
Note. The underlined bases denote T7 promoter sequences.
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