Transcriptional profiles of GSK-3β at different developmental stages. (A) RT-PCR shows presence of GSK-3β in all developmental stages. Lane L indicates 100 bp DNA ladder; lanes 1, 2, 3, 4, 5 and 6 represent GSK-3β present in egg, larva, nymph, adult, unfed midgut and fed midgut, respectively; (B) GSK-3β expression in larva, nymph, and adult stages; (C) GSK-3β expression in fed and unfed midguts of adult females; (D) Expression at different egg ages; (E) Knockdown of GSK-3β compared with the control group. The asterisk (*) denotes a significant difference compared with the control group as determined by Student’s t-test (p < 0.05) in case of B and D and One-way ANOVA with Bonferroni’s multiple comparison tests in case of A and C (p < 0.05).