Multilocus Enzyme Electrophoresis (MLEE) and hsp70 PCR-RFLP for Leishmania species identification. (A) Agarose gels stained for activity of 6-phosphogluconate dehydrogenase (6PGD) and glucose-6-phosphate dehydrogenase (G6PD) showing the patterns for Leishmania parasites isolated from Patients 1 and 2. (B) Polyacrylamide gel showing hsp70 products digested with HaeIII for Leishmania parasites isolated from Patients 1 and 2. In both assays, the profiles obtained for the two samples were compared to reference strains from different Leishmania species. For MLEE, the pattern was compatible with Leishmania (Viannia) lindenbergi. hsp70 PCR-RFLP was not useful for species identification of parasites from the two patients studied, as this marker cannot distinguish between L. (V.) lindenbergi and L. (V.) guyanensis. Lg = L. guyanensis, Lb = L. braziliensis, Lla = L. lainsoni, Ln = L. naiffi, Ls = L. shawi, Lu = L. utingensis, Lli = L. lindenbergi, and La = L. amazonensis; NC = Negative Control; MW = Molecular Weight