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Mature spermatozoon of Rhipidocotyle khalili. (a)–(f) Cross-sections in the anterior region of the spermatozoon showing the external ornamentation of the plasma membrane, a submembranous layer of cortical microtubules, the lateral expansion and a progressive appearance of the two axonemes. (g)–(m) Consecutives cross-sections in region II of the spermatozoon with only two axonemes and cortical microtubules (g)–(h) More posteriorly, disorganization and disappearance of the first axoneme (i)–(k) the progressive reduction of the number of cortical microtubules and the appearance of the mitochondrion (l) then the nucleus (M). (n)–(r) Consecutive cross-sections in the posterior region of the spermatozoon showing one axoneme, mitochondrion, nucleus and cortical microtubules (n); posterior extremity of the second axoneme, mitochondrion and nucleus (o); disappearance of the mitochondrion then of the axoneme 2 (p)–(q) Cross-section in the posterior extremity of the spermatozoon, showing only the nucleus (r). Aae1 = anterior extremity of the first axoneme, Aae2 = anterior extremity of the second axoneme, Ax1 = first axoneme, Ax2 = second axoneme, Ax = axoneme, Cm = cortical microtubules, Eo = external ornamentation, Le = lateral expansion, M = mitochondrion, Mi = microtubule, N = Nucleus, Pae1 = posterior extremity of the first axoneme, Pae2 = posterior extremity of the second axoneme. Scale Bars = 0.2 μm.
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