Figure 1
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Analysis of EtMIC2 expression in E. coli BL21. (A) Protein expression in bacterial pellets at different times of induction. Lane 1 protein marker; Lane 2 negative control (not induced with IPTG); Lanes 3, 4, 5, and 6 induced with IPTG at 2, 4, 6, and 8 h, respectively. (B) Protein expression in sonicated bacterial cells induced with IPTG at 8 h. S: supernatant, P: precipitate.
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