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Cytokine production, MHC expression, and apoptosis of antigen-presenting cells. RAW 264.7 macrophages (A), SRDC dendritic cells (A, B, C), or peritoneal exudate cells (PECs) (D) were stimulated for 24 h with 10 μg/mL of Tg-BE or Va-LE, or with DMSO as negative control. TNF-α (A) and NO (B) were quantified in supernatant by sandwich ELISA and the Griess method, respectively (n = 3). Expression of MHC molecules of classes I and II was measured by flow cytometry after labelling with specific antibodies (n = 2). Apoptosis was evaluated by flow cytometry after labelling with annexin V-FITC and propidium iodide (n = 3). Results are mean ± S.D. *p < 0.05 (Dunn’s multiple comparison test).
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