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Table 2
Limit of detection of the Illumigen Malaria plus LAMP method. Serial dilutions of Plasmodium falciparum in vitro cultivated clones 3D7 and W2 were tested using light microscopy (Giemsa stained thin blood smears), real-time PCR and LAMP Illumigen malaria. (inv. = invalid)
| Plasmodium falciparum clones | Parasitemia (dilutions from thin blood smears) | Real-time PCR (35 cycles) |
LAMP Illumigen Malaria (Meridian) |
||||
|---|---|---|---|---|---|---|---|
| % | Parasites /μL | Repeated measures | LAMP interpretation | ||||
| 3D7 | 0.002 | 100 | Positive | inv. | + | + | Positive |
| – | 0.0002 | 10 | Positive | + | + | + | Positive |
| – | 0.00002 | 1 | Positive | + | inv. | + | Positive |
| – | 0.000002 | 0.1 | Positive | + | − | + | Positive |
| – | 0.0000002 | 0.01 | Positive | − | − | − | Negative |
| – | 0.00000002 | 0.001 | Negative | − | − | − | Negative |
| – | 0.00000002 | 0.0001 | Negative | − | − | − | Negative |
| W2 | 0.002 | 100 | Positive | + | + | inv. | Positive |
| – | 0.0002 | 10 | Positive | + | + | inv. | Positive |
| – | 0.00002 | 1 | Positive | + | + | + | Positive |
| – | 0.000002 | 0.1 | Positive | + | + | + | Positive |
| – | 0.000002 | 0.01 | Positive | − | − | − | Negative |
| – | 0.0000002 | 0.001 | Negative | − | − | − | Negative |
| – | 0.00000002 | 0.0001 | Negative | − | − | − | Negative |
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