A) Detailed view on the splicing of Engam56_2. There are two alternative splice donor sites. Intron variants result in the occurrence of an alternative stop codon and a change of the encoded amino acid C-terminus. The intron of splicing variant 2 ranges from bp 1,329-1,473 and results in an alternative stop codon in the transcript, directing to an alternative C-terminal amino acid composition (→PIGGMPL). The intron of splicing variant 1 ranges from bp 1,363-1,473 and affects only the 3' UTR, but not the amino acid encoding region, which is consistent with the genomic open reading frame (→PIGGYGVYTWGPK). Splicing variant 2 was the predominant form found in the transcripts (ratio 4 to 1).

B) Graphical overview of splicing variants of Engam56_2. Indicated are the GenBank accession numbers and amino acids at the C-terminus of both variants, as well as the region of the intron.

C) Graphical overview of splicing of Engam56_1 shows predominantly a splicing variant, like shown for Engam56_2 splicing variant 2. The intron ranges from bp 1,368-1,512. No other splicing variant was found.