Egressed parasites displayed normal growth, infectivity and virulence. HFFs were infected with egressed tachyzoites (TNF-α-induced egress) or naturally egressed T. gondii. Twenty-four hours later, ten fields were randomly selected and the number of PV containing different tachyzoites was counted using an Olympus IX51 microscope. This experiment was repeated three times with similar results. C57BL/6 mice were infected intra-peritoneally with 103 tachyzoites and the survival rate was evaluated every day. Bars represent means ± SDs of five replicates. This experiment was repeated three times with similar results.
|No. of PV containing different tachyzoites (Tz)||No. of rosettes||Median survival time|
|2 Tz||4 Tz||8 Tz||16 Tz|
|Control||14.60 ± 3.97||20.80 ± 5.40||35.80 ± 8.87||3.40 ± 0.55||46.00 ± 5.56||5.5 days|
|Egressed||12.80 ± 1.92||17.20 ± 3.03||40.20 ± 9.42||3.60 ± 1.14||44.70 ± 5.72||5.5 days|
Current usage metrics show cumulative count of Article Views (full-text article views including HTML views, PDF and ePub downloads, according to the available data) and Abstracts Views on Vision4Press platform.
Data correspond to usage on the plateform after 2015. The current usage metrics is available 48-96 hours after online publication and is updated daily on week days.
Initial download of the metrics may take a while.