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TNF-α triggered egress of tachyzoites from infected HFFs. A and B, HFFs were infected with T. gondii (RH strain) for about 36 h, followed by treatment with 10 ng/mL TNF-α for 3 h or 6 h. Egressed parasites were calculated using FACS. C, HFFs were infected with T. gondii (RH strain) for different time periods followed by TNF-α treatment (10 ng/mL, 6 h) and an egress assay. Bars represent means ± SDs of five replicates. This experiment was repeated three times with similar results, *p < 0.05. Bar = 50 µm.

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