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1 – Sand fly eggs are dislodged from the plaster surface in the bottom of an ovipot using a Waterpik® (oral irrigator) with fan-spray attachment; 2 – The egg/water suspension is flushed into the appropriate colour-coded sieve; 3 – Eggs in a sieve that have been flushed from an ovipot; 4 – The sieve containing eggs is nested in a soaking pan filled with 1% sodium hypochlorite; 5 – After soaking, the eggs are flushed to one side of the sieve with a squirt bottle; 6 – The eggs are flushed from the sieve and distributed evenly over the plaster surface in the bottom of a clean ovipot. (Photos by T. Rowland).
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