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Research Article

Table 1

Identification of unsporulated Eimeria tenella oocyst proteins in non-redundant sequence databases (NCBI) using data from MALDI-TOF-MS and MALDI-TOF/TOF-MS.

Spot Accession number Score Peptide matched Sequence coverage (%) Mr/pI Protein
3 gi|25989637 105 17/79 38 36232/7.00 lactate dehydrogenase [E. acervulina]
4 gi|145535758 72 26/93 22 155774/5.61 hypothetical protein GSPATT00019908001 (P. t)
6 gi|156097314 65 19/83 19 159966/6.14 6-phosphofructokinase, putative (P. v)
7 gi|118490906 154 12/37 50 27839/9.31 hypothetical protein (E. tenella)
8 gi|118490770 317 42/96 40 84827/8.27 hypothetical protein (E. tenella)
10 gi|124804156 64 16/86 19 84877/9.22 hypothetical protein PF11_0247 (P .f)
11 gi|50400241 230 32/100 69 48458/5.62 Enolase (E. tenella)
13 gi|25989639 136 22/100 54 35399/6.54 lactate dehydrogenase (E. tenella)
16 gi|118397285 79 21/96 27 126785/7.55 hypothetical protein TTHERM_00947400 (T. t)
20 gi|401829 427 46/76 56 70814/5.16 heat-shock protein [E. acervulina]
21 gi|145517049 71 16/91 35 46979/9.32 hypothetical protein GSPATT00012541001 (P. t)
22 gi|401829 86 19/98 27 70814/5.16 heat- shock protein [E. acervulina]
23 gi|401829 605 66/98 67 70814/5.16 heat-shock protein [E. acervulina]
24 gi|50400241 260 30/89 64 48458/5.62 Enolase (E. tenella)
26 gi|124054715 215 17/50 66 13343/5.92 actin depolymerizing factor (E. tenella)
28 gi|1037176 269 39/78 29 77516/5.22 immunoglobulin heavy chain binding protein (E. tenella)
31 gi|118379641 66 17/88 20 106631/6.01 hypothetical protein TTHERM_00348310 (T. t)
32 gi|118377447 74 16/92 22 87148/6.14 hypothetical protein TTHERM_01207560 (T. t)
33 gi|4033429 112 22/92 43 58238/5.95 Pyruvate kinase (PK) (E. tenella)
34 gi|145494414 69 10/96 42 22764/9.18 hypothetical protein GSPATT00006303001 (P. t)
35 gi|118359856 77 14/94 23 70549/8.46 polyadenylate-binding protein 2 (T. t)
36 gi|4033429 146 24/72 46 58238/5.95 Pyruvate kinase (PK) (E. tenella)
38 gi|145519139 77 25/93 18 192289/5.98 hypothetical protein GSPATT00013486001 (P. t)
39 gi|124805406 70 11/97 34 37233/9.54 mitochondrial phosphate carrier protein (P. f)
40 gi|145498869 76 15/96 38 47850/10.02 hypothetical protein GSPATT00036807001 (P. t)
41 gi|50400241 132 23/100 48 48458/5.62 Enolase (E. tenella)
43 gi|21542248 214 33/94 62 50377/4.78 Tubulin beta chain (Beta-tubulin) (E. tenella)
44 gi|156098346 67 10/52 14 123864/9.42 hypothetical protein PVX_091055 (P. v)
45 gi|50400241 204 26/63 59 48458/5.62 Enolase (E. tenella)
46 gi|50400241 197 26/67 59 48458/5.62 Enolase (E. tenella)

Note: P. t: Paramecium tetraurelia strain d4-2; T. t: Tetrahymena thermophila SB210; P. f: Plasmodium falciparum 3D7; P. v: Plasmodium vivax SaI-1.* common protein in two stages

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