Immunoproteomic analysis of the protein repertoire of unsporulated Eimeria tenella oocysts

Open Access

Table 1

Identification of unsporulated Eimeria tenella oocyst proteins in non-redundant sequence databases (NCBI) using data from MALDI-TOF-MS and MALDI-TOF/TOF-MS.

Spot	Accession number	Score	Peptide matched	Sequence coverage (%)	Mr/pI	Protein
3	gi\|25989637	105	17/79	38	36232/7.00	lactate dehydrogenase [E. acervulina]
4	gi\|145535758	72	26/93	22	155774/5.61	hypothetical protein GSPATT00019908001 (P. t)
6	gi\|156097314	65	19/83	19	159966/6.14	6-phosphofructokinase, putative (P. v)
7	gi\|118490906	154	12/37	50	27839/9.31	hypothetical protein (E. tenella)
8	gi\|118490770	317	42/96	40	84827/8.27	hypothetical protein (E. tenella)
10	gi\|124804156	64	16/86	19	84877/9.22	hypothetical protein PF11_0247 (P .f)
11	gi\|50400241	230	32/100	69	48458/5.62	Enolase (E. tenella)
13	gi\|25989639	136	22/100	54	35399/6.54	lactate dehydrogenase (E. tenella)
16	gi\|118397285	79	21/96	27	126785/7.55	hypothetical protein TTHERM_00947400 (T. t)
20	gi\|401829	427	46/76	56	70814/5.16	heat-shock protein [E. acervulina]
21	gi\|145517049	71	16/91	35	46979/9.32	hypothetical protein GSPATT00012541001 (P. t)
22	gi\|401829	86	19/98	27	70814/5.16	heat- shock protein [E. acervulina]
23	gi\|401829	605	66/98	67	70814/5.16	heat-shock protein [E. acervulina]
24	gi\|50400241	260	30/89	64	48458/5.62	Enolase (E. tenella)
26	gi\|124054715	215	17/50	66	13343/5.92	actin depolymerizing factor (E. tenella)
28	gi\|1037176	269	39/78	29	77516/5.22	immunoglobulin heavy chain binding protein (E. tenella)
31	gi\|118379641	66	17/88	20	106631/6.01	hypothetical protein TTHERM_00348310 (T. t)
32	gi\|118377447	74	16/92	22	87148/6.14	hypothetical protein TTHERM_01207560 (T. t)
33	gi\|4033429	112	22/92	43	58238/5.95	Pyruvate kinase (PK) (E. tenella)
34	gi\|145494414	69	10/96	42	22764/9.18	hypothetical protein GSPATT00006303001 (P. t)
35	gi\|118359856	77	14/94	23	70549/8.46	polyadenylate-binding protein 2 (T. t)
36	gi\|4033429	146	24/72	46	58238/5.95	Pyruvate kinase (PK) (E. tenella)
38	gi\|145519139	77	25/93	18	192289/5.98	hypothetical protein GSPATT00013486001 (P. t)
39	gi\|124805406	70	11/97	34	37233/9.54	mitochondrial phosphate carrier protein (P. f)
40	gi\|145498869	76	15/96	38	47850/10.02	hypothetical protein GSPATT00036807001 (P. t)
41	gi\|50400241	132	23/100	48	48458/5.62	Enolase (E. tenella)
43	gi\|21542248	214	33/94	62	50377/4.78	Tubulin beta chain (Beta-tubulin) (E. tenella)
44	gi\|156098346	67	10/52	14	123864/9.42	hypothetical protein PVX_091055 (P. v)
45	gi\|50400241	204	26/63	59	48458/5.62	Enolase (E. tenella)
46	gi\|50400241	197	26/67	59	48458/5.62	Enolase (E. tenella)

Note: P. t: Paramecium tetraurelia strain d4-2; T. t: Tetrahymena thermophila SB210; P. f: Plasmodium falciparum 3D7; P. v: Plasmodium vivax SaI-1.* common protein in two stages

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