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Histological analysis of spleen tissue sections in acutely Trypanosoma cruzi infected mice, treated with astaxanthin and/or nifurtimox. Spleen tissue sections (5 μm) were obtained at 60 days post-infection or at the time the animals died due to infection, and stained with hematoxylin-eosin. Representative micrographs of mice (BALB/c) from the following groups are shown: (A) G1 (Tc); (B) G2 (Tc/ASTX); (C) G5 (saline solution); (D) G8 (ASTX). The micrograph from G5 could represent all groups from G3 to G7; all of them were considered histologically normal. White arrow: lymphoid follicles; Black arrow: red pulp.
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