Histological analysis of heart tissue sections from acutely Trypanosoma cruzi infected mice, treated with astaxanthin and/or nifurtimox. Heart tissue sections from the left ventricle were processed on the day animals died (either due to infection or when euthanized on day 60 post-infection). Tissue sections (5 μm) were stained with hematoxylin-eosin. Representative micrographs are shown for mice from the following groups: (A) G1 (Tc); (B) G2 (Tc/ASTX); (C) G5 (saline solution); (D) G8 (ASTX). The micrograph from G5 could represent all groups from G3 to G7; all of them were considered histologically normal. Black arrow, amastigote nests; white arrow, lymphoplasmacytic infiltrate. (400× amplification).