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Western blot analyses for antigenic specificity of immunized mouse sera. Four weeks after immunization, TsoAg was probed with sera from mice immunized with one dose of PLG-rSAG1/2 MPs (lane 1), one dose of rSAG1/2 (Vet L-10) (lane 2), two doses of rSAG1/2 (Vet L-10) (lane 3), one dose of rSAG1/2 alone (lane 4), one dose of blank PLG (lane 5) or PBS (lane 6). In addition, the mouse mAbs TG-1 (lane 7) and TG-2 (lane 8) were also used as markers for SAG1 and SAG2, respectively. Standard protein markers (lane M) are shown on the left.
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